Construction services sector is one of the many areas of business are classified as highly vulnerable to accidents or exposure to occupational disease. Compliance of workers in the use of personal protective equipment (PPE) can reduce the risk of accidents or occupational diseases. This study aims to determine the relationship of knowledge, perceptions, attitudes, availability of PPE, comfort, and supervision of the use of PPE in construction workers of the AP Pettarani flyover toll road in Makassar City. This type of research is observational analytic with a cross sectional study approach. Data collection was conducted in March - April 2019 for 63 workers as samples taken using the proportionate stratified sampling technique. Data obtained using a questionnaire. Data were analyzed by univariate and bivariate using the chi square test on SPSS applications. The results showed that there was a relationship between knowledge p = 0,036 (p <0,05), perception p = 0,035 (p <0,05), attitude p = 0,010 (p <0,05), PPE availability p = 0,020 (p <0,05) with APD comfort while variable p = 1,000 (p> 0,05) and control p = 1,000 (p> 0,05) was not associated with the use of PPE. The conclusion of this research is that there is a relation between the knowledge, perceptions, attitudes, availibity of PPE with the use of PPE.