EAS Publisher | Isolation of Podoviridae Bacteriophages of Pseudomonas Aeruginosa from Hospital Sewage Water and the River Ganges

Isolation of Podoviridae Bacteriophages of Pseudomonas Aeruginosa from Hospital Sewage Water and the River Ganges

DOI : https://doi.org/10.36349/easjbg.2026.v08i03.001

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The effectiveness of conventional antibiotic regimens has been severely compromised by the rapid dissemination of the multidrug-resistant (MDR) Pseudomonas aeruginosa, a critical ESKAPE pathogen characterized by impermeable outer membranes, active efflux mechanism, and robustbiofilm matrix production. Lytic bacteriophages therapy represents a highly promising alternative treatment approach. In this study, we isolated and characterized virulent bacteriophages targeting a MDR P.aeruginosahost from hospital sewage water and the River Ganges, and for this we adapted the host-mediated selective enrichment and double layer agar typing methodologies that was first developed by Dr. Sachindranath Mukherjee. The isolated phages generated homogenous, transparent plaques ranging from 2.5 to 4.5 mm in a diameter. The absence of central turbidity or peripheral halo zones confirms a lytic, obligate lifecycle. These findings demonstrates that bioprospecting geographically diverse environmental reservoirs to produce lytic phages for downstream lyophilization, encapsulation against chronic nosocomial infections.

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