Background: Haemostasis, inflammation, proliferation, and remodeling represent the various stages of the wound healing process. Insufficient blood circulation to the wound site and the presence of microbial infections are two additional factors that can impede healing. Improving both the systemic and local health of the patient, along with establishing an optimal environment for healing, are crucial elements of effective wound management. Various products have been created to modify the wound environment, aiming to establish a safe, moist, and infection-free milieu for recovery. In the wound healing cascade, newer materials are being introduced to complement or replace older substrates. Methodology: Carbopol 940 (Carbomer Homopolymer USP), Disodium Edetate IP, Sodium Methylparaben IP, Sodium Propylparaben IP, and Sodium Hydroxide IP were used to manufacture the Polyherbal Gel, which was then tested for wound healing activity. For evaluation of wound healing potential of different Polyherbal gel formulations various parameters like wound contraction, tensile strength and Hydroxyproline as a biomarker were assayed. Result: It has been observed that animals given the F1 and F3 formulations experienced the quickest wound healing. It was discovered that the epithelization duration of F1 and F3 was essentially typical. The incision wound models demonstrated that, when compared to the standard, the mean tensile strength of the recovered incision wound on the tenth post-injury day showed F1 & F3 formulation to be most significant. Conclusion: The investigation's results showed that the synergistic effects of flavonoid and plant phenolic present in the extracts, the results of the current study demonstrated that polyherbal formulations containing ethanolic leaf extract of M. oleifera, O. sanctum, and A. indica showed potent wound healing efficacy.