Introduction: Human Immunodeficiency Virus (HIV) and Acquired Immune Deficiency Syndrome (AIDS) remain significant public health challenges globally, with cultural and social stigma, and discrimination continuing to affect the quality of life of people living with the condition. While health workers play a direct role in HIV care, non-health workers within hospital environments also interact with patients and can influence their experience and wellbeing. This study assesses the awareness, perception and attitude of non-health workers towards patients with HIV/AIDS in Rivers State University Teaching Hospital. Method: A descriptive cross-sectional research design was employed, using the a self-structured APAHQ questionnaire. A simple random sampling technique was used to derive data from 121 non-health workers at the Rivers State University Teaching Hospital. Data was analyzed using descriptive statistics of mean, frequency and percentages. Results: The findings of the study revealed a generally high level of awareness about HIV/AIDS at 94.3%. The mean perception of 3.2 ± 0.6 indicates a moderate perception of the respondents about patients with HIV/AIDS. The study showed a significant negative attitude of respondents towards patients with HIV/AIDS at Rivers State University Teaching Hospital with mean value 0.3 ± 0.7. Conclusions: This study emphasized the need for continuous workplace education and sensitization campaigns among non-health workers to clear myths and misconceptions of HIV/AIDS in order to end stigmatization of patients with HIV/AIDS. Based on the findings, the study recommended that public enlightenment, strengthening HIV literacy and empathy among non-health workers will foster a more supportive and inclusive hospital environments for patients living with HIV/AIDS.