In the present work, an ethanolic extract of A. digitata has been tested in vitro against the bovine parasite Onchocerca ochengi and Caenorhabditis elegans. The both nematods were incubated in different concentrations of the plant extract solubilized in RPMI 1640 supplemented with penicillin/streptomycin (100 U/mL/100µg/mL) and on NGM-agar. Ivermectin, Levamizole were used as the positive controls. The worm viability was determined by MTT/formazan colorimetry method after 24, 48 and 72 h. The oral acute toxicity on rats (60-120g) of the fruit pulp and its phytochemical composition were also assessed. The LC50 values were determined using Probit Analysis and data were expressed as mean ± standard deviation. The extract was active on macrofilariae of O. ochengi and all C. elegans living strains. On O. ochengi, the LC50 values for ethanolic FPE were 36.01, 12.03 and 5.05 µg/mL respectively after 24, 48 and 72 h. C. elegans strains were sensible to the FPE. The calculated LC50 were 82.08, 60.02 and 49.01 µg/mL for the wild-type; 209.03, 151.21 and 95.61 µg/mL for CB211 strain; 370.10, 224.0.3 and 210.06 for VC722 strain respectively after 24, 48 and 72 h. Among the phytochemical constituents of the fruit pulp tannins (43.88 mg/kg GAE) were the most abundant followed by phenolic acid (21.28 mg/kg GAE), saponins (12.99 mg/kg) and flavonoids (0.07 mg of rutin/100g). The oral acute toxicity of FPE assessed on rats showed that the LD50 was above 5000 mg/kg suggesting no toxicity. The relative organ weights of liver of rats sacrificed for all group varied from to 3.94 ± 0.23g to 12.92 ± 0.61g. The group treated with CCL4 showed the highest values of relative organ weight with a maximum of 12.92 ± 0.61g for males and 12.78 ± 0.54g females. The difference is significant (p-value = 0.001) between the ALAT and ASAT values founded in liver of rats treated with FPE when compared to those treated with CCL4 (carbon tetrachloride), ivermectin and levamisole. However, not significant d