Chicken coccidiosis is a protozoan disease that significantly impacts poultry globally. The disease is caused by Eimeria species, which causes intestinal damage leading to reduced growth, diarrhea, and death in severe cases. Control strategy includes the use of anticoccidial drugs, vaccination, and good biosecurity. A cross-sectional study was conducted in Kikuyu Sub-County, Kiambu County, Kenya, from 28th October, 2021, to 8th November,2021, to assess the farmers’ knowledge, attitude, and practices (KAP) on chicken coccidiosis and collect fecal samples for estimation of prevalence, intensity of infections, and speciation Eimeria species involved. Additionally, a trial was undertaken to test the efficacy of commercial anticoccidials drug in the market. A semi-structured questionnaire was used to assess farmers’ KAP. Modified McMaster technique was used to analyze fecal samples for oocyst per gram (opg) of feces and positive fecal samples were cultured and sporulated oocyst examined microscopically to identify and characterize probable Eimeria species. One hundred- and twenty-five-day old chicks were acclimatized for 13 days then randomly assigned into five treatment groups; amprolium (GA3), toltrazuril (GT4), sulphachloropyrazine (GS5), negative (GN1) and positive (GP2) controls. After 24-hour of starvation, each chick in GA3, GT4, GS5 and GP2 was orally inoculated with 3.0 x 105 sporulated oocysts. Anticoccidial treatments based on the manufacturers’ recommended dosage commenced when birds showed clinical signs typical of coccidiosis. Effects of treatment was monitored for 5 days through clinical observations and fecal oocyst counts. Experiment was terminated 10th day post infection (dpi). Data was analyzed through descriptive statistics, and one way ANOVA. A total of 48 farms; 10 in Sigona, 10 in Karai, 10 in Nachu, 10 in Kikuyu and 8 in Kinoo wards were used in the study.