Background: Job dissatisfaction and nurses’ intention to leave are an increasing problem that threatens the function of the health care sector worldwide, especially in developing countries, including Tanzania. The aim of this study was to determine and compare nurses’ job satisfaction and intention to leave their job and the associated factors in public and private healthcare facilities in Dar es Salaam, Tanzania. Methods: This was a cross-sectional survey that was conducted from January to August 2023 to determine nurses’ job satisfaction and intention to leave their job and associated factors in public and private healthcare facilities in Dar es Salaam, Tanzania. A multistage cluster sampling technique was employed to obtain the required number of the study population. Results: A total of 222 respondents were studied. Of these, 126(56.8%) were satisfied with their job and the remaining 96(43.2%) were not satisfied. Additionally, out of 222 respondents, 132(59.5%) were intended to leave their job and the remaining 90(40.5%) were not intended to leave their job. Nurses aged >50 years were 3.54 more likely to be satisfied with their job (AOR: 3.54, 95% CI (2.44-8.55), p= 0.002) compared to nurses less than 30 years of age and were less likely intended to leave their nursing profession (AOR: 9.22, 95% CI (2.32-14.33), p<0.001). Bachelor or master's degree holder nurses were found to be satisfied with their job (AOR: 4.77, 95% CI (2.61-12.43), p= 0.001) and were less likely intended to leave their job (AOR: 4.83, 95% CI (1.73-13.50), p= 0.002) compared with certificate and diploma holder nurses. Nurses working in private facilities showed higher level of job satisfaction (AOR: 3.11, 95% CI (2.38-8.14), p<0.001) and were less likely intend to leave their job (AOR: 9.76, 95% CI (3.44-23.11), p=0.001) compared to nurses working in public health facilities. Nurses with work experience of 10 years and above were found to be satisfied with their job (AOR: 3.46, 95% CI (2.71-