Background: Anaesthesia is essential for successful surgical interventions to achieve complete immobilization, analgesia, muscle relaxation and unconsciousness. It facilitates pain-free surgical. Ketamine and lignocaine are examples of anaesthetic agents, often administered alone or in combination, for instance with anticholinergics or adrenaline, respectively. However, their effects on hemostatic and biochemical parameters are still subject of ongoing research. In this study, hemostatic parameters such as bleeding time, prothrombin time, activated partial thromboplastin time of male wistar rats treated with ketamine and different kinds of lignocaine were explored. Methodology: 35 male wistar rats were used for the study. These animals were housed for one week for them to acclimatized before being sacrificed. Blood samples were collected using sample bottles and appropriate laboratory techniques were employed for laboratory investigations. Data obtained from this study was analysed using one way ANOVA. Discussion: Ketamine and lidocaine when used both alone and in combination caused substantial variations in the haematological and biochemical parameters with the decrease in haemoglobin, level increase in erythropoietin and potassium, and an increase in plasma viscosity. Nevertheless, haemostatic parameters and erythrocyte integrity did not change, which means that there was low risk of coagulation. These implications present the possibility of renal and cardiovascular consequences that need a careful clinical application. Conclusion: It is concluded that two different kinds of anaesthesia does not alter hemostatic cell lines and hence cannot cause hypercoagulation and hypocoagulation in users. Use of anaesthesia for surgical intervention is encouraged.