Background: Coronary artery disease ("CAD") is the most frequent kind of cardiovascular disease and one of the primary causes of morbidity and death in different cultures. Several risk factors for this condition have been found so far. For many years, researchers have researched problems such as old age, gender, smoking, diabetes mellitus, hyperlipidemia, higher body mass index, and a good family history. Various research on the risk factors for this illness have shown disparate and conflicting findings, suggesting that achieving a consistent and rational reaction might aid in the prevention and treatment of cardiovascular disease. Although identifying risk factors has the potential to lower CAD dramatically. Several genes and associated proteins have been recognized in relation to cardiovascular illness. The purpose of this research was to identify and compare the expression of the VEGF gene in the positive angiography and control groups. Method: The target group is 20 people, 10 of whom are healthy and 10 of whom have cardiovascular disease who undergo angiography. In this work, RNA was extracted from the samples, cDNA was produced, and Real Time PCR was used to quantify and qualitatively measure gene expression. SPSS was used to do statistical analysis. Considered significant was a significance level of.05. Results: Based on quantitative data obtained from Real Time PCR, VEGF expression was significantly increased in the angio-positive group compared to the "control group" (P <0.05). Statistical analysis showed a significant relationship between demographic factors Current smoker, DM, HTN, Education level, Male, Age and vascular stenosis (P> 0.05) There was also a significant relationship between biochemical factors LDL-C, HDL-c, Triglyceride, Total cholesterol, No FBS and stenosis (P> 0.05). Conclusion: This study showed that the expression of VEGF in people with coronary artery disease is statistically significantly increased. It also showed that coronary arter