In light of global commitments to eradicate poliovirus and ensure strict laboratory containment, Zambia conducted a nationwide laboratory inventory and survey in November 2024 to identify facilities handling or retaining poliovirus infectious materials (IMs) or potentially infectious materials (PIMs). This effort followed previous surveys conducted in 2015 and 2019 (WHO, 2020), amid recurring vaccine-derived poliovirus (cVDPV2) outbreaks and cross-border risks (WHO, 2021). A total of 42 laboratories across all 10 provinces were surveyed using the Open Data Kit (ODK) tool (CDC, 2020). Of these, 18 laboratories reported handling samples classified as PIMs, and three facilities UTH Virology, ZNPHRL, and TDRC were found to retain 95,455 PIMs (including stool and nasopharyngeal samples), while no facility retained infectious materials (IMs). The UTH Virology Lab remains the only WHO-accredited national polio lab authorized to retain such samples (WHO, 2015). Data were collected and analyzed using SQL Server and GIS tools, providing detailed insights into material distribution and lab categorization. Despite challenges with survey tool limitations and access to centralized databases, the findings underscore Zambia's continued vigilance in poliovirus containment, with only 0.03% of unusable OPV2 vials unaccounted for (WHO, 2021). The survey confirms that Zambia maintains a low risk of poliovirus spread and emphasizes the need for periodic verification visits, refined survey tools, and real-time inventory tracking to uphold global containment standards (Matapo, 2012).