Background: Psychological stress is the body's natural response to physical or emotional pressure, leading to the release of hormones like cortisol and adrenaline. While stress can sometimes be beneficial by enhancing focus and decision-making, prolonged stress can negatively impact mental and physical health. To reduce its effects, practicing exercise, relaxation techniques, and effective time management is recommended. Objective: To Evaluate nurse’s knowledge Among Psychological stress in pediatric department and Nursery intensive care unit Methods: This descriptive cross sectional hospital-based study design to assess stress among nurses in the pediatric department and NICU at Almak Nimr Hospital in period extended from "December to February 2025" purposive sampling technique was used, with a sample size of 38 nurses. Data were collected using a closed-ended questionnaire designed by the researchers. The questionnaire consisted of 18 questions finally the data was analyzed by statistical package for social solution (SPSS) version 18 then the data represents in form tables and figures. Results: The results of present study clarified that less than half (48%) of the study group experienced stress due to workload, (16%) due to a lack of medical resources, (10%) due to dealing with critical cases, and conflicts with colleagues and patients, (16%) indicated that all the mentioned factors contributed to their work-related stress. Also about Impact of anxiety or stress affect the quality of professional performance more than half (52%) of study group had moderate impact, (16%) of study group had significant impact, (32%) of study group had minor impact. And about dealing with psychological stress the result showed that the majority (84%) of study group had poor knowledge, only (2%) of study group had good knowledge, and (14%) of study group had fair knowledge. Conclusion: The result concluded that the nurses knowledge regarding physical health effect of stress is (82%)