This study was to make bio-fertilizers with Azotobacteria and Azospirilum consortiums and to test the influence of bio-fertilizers on Okra plant growth. This investigation was conducted using the soil from the Biological Garden of the University of Cross River State, Calabar. Pure culture and mass production was performed in batch fermentation at optimum condition by means of specific medium for Azospirilum and Azotobacter. Organisms were isolated from the soil samples and confirmed using biochemical tests. The bio-fertilizer effect on the growth parameters of Okra plants was carried out on Day 21. The post germination, phenotypic and observations showed significant differences in performance between plant length, mass, root numbers, root length, total plant length, breadth of leaves, shoot length, root mass and biological yield. The biochemical indices exibited a significant rise (P < 0.05) in chlorophyll content (mg/g.fr. wt) as compared to Control (0.010±0.000), Azospirilum (0.016±0.000), Azotobacter (0.016±0.000) and consortium (0.018±0.000). Significant rise (P<0.05) in carbohydrate content (%) control (51.976±0.768), Azospirilum (69.690±3), Azotobacter (69).656+2), and consortium 70).190± (5). Protein content control (0.028±0.001), Azospirilum (0.040±0.000), Azotobacter (0.060±0.001) and consortium (0.061±0.000) all significantly increased [P< 0.05]. Growth is significantly affected by single injection and control when compared to plants being treated with Azotobacter, Azospirilum or both (P < 0.05). According to the findings, plant mineral fixation through nitrogen fixation and phosphorous solubilization seems to be improved by bio-fertilizer inoculation as well as their mineral nutrition’s improvement.