Use of simple sugars, artificial and processed sweeteners enhances the risk of diabetes and obesity. People were unaware of benefits of natural sweeteners. An experimental study based on sample size of 10 participants was conducted, to find out the glycemic response of natural sweeteners like sugarcane juice, honey and jaggery in healthy individuals. They were instructed to consume all the juice served in a period of 5 min. Further blood samples were taken at 0, 30, 60, 90, 120 and 150 minutes after consumption. Participants were remained sedentary during each session. Blood was obtained by finger-prick and tested by the glucometer. Dextrose means values 91.3, 138.5, 116.7, 96.2, 88.3, 80.9. Honey mean values 84.5 115.5 96.1 79.7 72.7 75.4. Jaggery mean values 87.4, 129.6, 103.1, 90.4, 86, 80.7. Sugarcane juice means values 83.3, 102.1, 97.4, 91.9, 76.6, 74.6 at 0, 30, 60, 90, 120, 150 minutes respectively. In conclusion, detailed study of glycemic values of dextrose, honey, jaggery and sugarcane juice shows that all are good glycemic substances. So, healthy persons can be recommended to use natural sweeteners because of their good glycemic response and nutrient value. Being natural sweeteners (honey, jaggery, sugarcane) they have pure nutrient value and they boost up blood sugar level energizing the body to carry out the daily activities in a healthy way without making people obese.