Streptomyces sp. play important role in controlling pathogenic bacteria by using different mechanisms so this study aims to diagnose the Streptomyces sp. and select strains having the ability to inhibit biofilm-forming bacteria and degradation proteins and starch. The capacity of bacteria to produce biofilms was examined by staining with 1% crystal violet. The ability to inhibit biofilm-forming bacteria, proteolytic and amylolytic, were determined by bacterial drop and well diffusion methods. The bacteria capable of creating biofilms were isolated from urinary tract infection and identified by biochemical reactions and sequencing the 16S rRNA gene. The Selected 6 Streptomyces strains which were isolated from soil are capable of inhibiting the growth of Escherichia coli and capable of degrading protein and starch. The strain STV1 was selected for 16S rRNA gene sequencing with standard isolates. Isolation of Escherichia coli capable of forming biofilm from urinary tract infection. Selection of Streptomyces sp strains capable of inhibiting biofilm-forming bacteria. The research is the first step to studying the application of Streptomyces sp in inhibiting biofilm-forming bacteria and in treating organic matter.