This study aims to analyze the factors that influence Burnout Syndrome in health workers at the Batakte Health Center, Kupang Regency. The method used is a quantitative research method with a cross sectional design. The sampling technique uses total sampling. The results showed that the results of the chi-square analysis showed no effect of age (p = 0.771), gender (p = 0.375), education (p = 1.000) on the incidence of burnout syndrome in health workers in the Working Area of the Batakte Health Center, Kupang Regency. There is an influence of marital status (p = 0.033), length of work (p = 0.002), workload (p = 0.001), social support (p = 0.002) on the incidence of burnout syndrome in health workers in the Working Area of the Batakte Health Center, Kupang Regency. Simultaneously, the variables of length of work (OR 6.352), workload (OR 6.647) and social support (OR 4.555) have an influence on the incidence of burnout syndrome in health workers in the Working Area of the Batakte Health Center, Kupang Regency. The workload variable is the most dominant variable that has an influence on the incidence of burnout syndrome in health workers with OR (7.163), p=0.000, CI=2.466-20.808. In conclusion, the factors that influence Burnout Syndrome in health workers are workload.