Due to increasing drug resistance bacterial isolates in hospitals, morbidity and mortality are gradually increasing throughout the globe. Newer antibiotics are also not coming up as most drug manufacturing industries are reluctant to manufacture antibiotics due to low profit. In this context, we have to determine the antimicrobial activities of other common chemical agents. Thus in this experiment, we have studied L-asparagine- an amino acid with various biological activities to find out its antimicrobial activity if any. We were particularly interested in MDR bacteria in this study. Clinically isolated bacteria from different samples were identified by the automated Vitek automated system and stocked in the laboratory for our study. All isolates were used in lawn culture to find out the sensitivity of different bacteria to asparagine. In our experiment, we aimed to find out which specific concentration of L-Asparagine amino acid shows the most potent antibacterial effect on these bacterial species. In general, growths of all tested bacteria found inhibited at a very low concentration of 39 µg/ml. This uniform result against all resistant bacteria tested in this experiment clearly indicates its future utility against all extremely drug resistant bacteria.