Background: Blood transfusion is essential and the most common therapeutic use in which transfused blood and its elements to save a patient's life. However, transfusion recovers a patient's life, but it is not without risk. Blood transfusion is a critical and standard therapeutic procedure in which patients are given blood and its components to preserve their lives. Millions of patients want blood and blood merchandise transfusions worldwide. Blood infusions may be a very important want for a few patients; however, while not cautious, it may be a grievous intervention. It is calculated that in every 13000 cases of Blood infusions, one error happens mainly because of preventable human errors through applicable education and reform in insertion protocols. Objective: The aim was to assess the level of Nurses' Knowledge Regarding the Management of Blood Transfusion patients at 250 Bedded Mohammad Ali Hospital, Bogura. Methodology: This descriptive type of cross-sectional study design was used, and a 110-sample size that was a purposive sampling technique followed those who met the inclusion criteria to assess the Nurses' Knowledge Regarding the Management of Blood Transfusion. The study was conducted from July 2021 to December 2021. The instruments for data collection were a semi-structured questionnaire composed of two parts: Demographic variables and a nurses' knowledge-related questionnaire. The respondents were assured that confidentially and anonymity were strictly maintained. Results: The findings of the present study revealed that 12.73% had a high level of knowledge score, 47.27% had a moderate level of knowledge, and 40% had a low level of knowledge regarding blood transfusion. Conclusion: It is concluded that the study provided baseline information for the current situations of the selected hospital. Nurses play an integral role in blood transfusion. Also, nurses must have adequate knowledge of all transfusion processes to maintain patients' safety. ........