EAS Publisher | Comparative Assessment of the Effect of EDTA, Lactic acid and Citric acid on Root Dentin Microhardness

Comparative Assessment of the Effect of EDTA, Lactic acid and Citric acid on Root Dentin Microhardness

DOI : https://doi.org/10.36349/easjdom.2023.v05i06.002

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Aim: To determine the effect of ethylenediaminetetraacetic acid (EDTA), lactic acid and citric acid on root dentin microhardness. Materials and Methods: In this in vitro study, twenty freshly extracted anterior teeth were transversely sectioned at the level of cementoenamel junction. The middle third of each root is then horizontally sectioned into slices that are 4 mm thick to obtain a total of 20 dentin discs. Based on the chelating agent used, the samples were randomly divided into four groups- saline (control group), 17% EDTA, 20% lactic acid and 10% citric acid. Vicker’s indenter was used to test the microhardness of the dentin before and after treatment with the chelating agents for two minutes. Data was obtained and statistically evaluated using a one-way analysis of variance (ANOVA), paired – t test and Tukey’s post hoc test. Results: The microhardness values varied significantly among the groups. Citric acid and and lactic acid were less effective at reducing dentin microhardness. EDTA led to a higher reduction in microhardness. Conclusion: All the chelating solutions reduced the microhardness. Lactic acid showed less alteration in the hardness of root dentin than EDTA.

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