Physical Education Major in Colleges and universities is the main force to cultivate excellent physical education reserve talents in China. In order to improve the training quality of compound talents in physical education, this study takes the curriculum of physical education major in colleges and universities as the research object, and studies the curriculum structure, curriculum teaching mode, curriculum practice and curriculum evaluation of the curriculum of physical education major. The research shows that optimizing physical education curriculum based on OBE concept can meet the needs of physical education curriculum construction, deepen the curriculum reform of physical education, and help achieve the goal of training physical education professionals. However, based on the current research results, it is found that there are corresponding deficiencies in the implementation of each part. Therefore, this study puts forward the corresponding reform strategies according to the five contents emphasized by the OBE concept: everyone can succeed, personalized evaluation, proficiency, performance responsibility and ability standard. For the course structure, we should increase the types of elective courses and improve the proportion of technical courses; For the classroom teaching mode, adopt the flexible organization form of the course, update the design structure of the course and enrich the teaching methods of the course; For curriculum practice, we should pay attention to curriculum teaching practice and strengthen educational practice; For curriculum evaluation, comprehensive evaluation content and diversified evaluation methods are adopted.