This study examined the investment in non-current assets and the performance of quoted manufacturing firms in Nigeria. Secondary data were collected from annual reports and accounts of the fifteen (15) selected quoted firms for the period of eight (8) years spanning from 2012 to 2019. Data collected were analysed using descriptive statistics, correlation and regression analyses. The empirical results revealed that investment in tangible non-current assets has positive and significant effect on the return on assets (ROA) of the selected manufacturing firms as confirmed by the coefficient and probability value of 0.95(P=0.000). Investment in intangible non-current assets also has positive and significant effect on the return on assets with the coefficient and probability value of 0.44(P=0.000); debt to assets ratio has a positive and significant effect on return on assets; while assets turnover ratio has negative but insignificant effect on return on assets. The overall coefficient of determination, adjusted (R2) of 0.923 showed that about 92% of the total variation in the ROA is explained by investment in Tangible Non- Current Asset (TNCA), Intangible Non-Current Assets (INCA), Debt-Assets Ratio (DAR) and Assets Turnover Ratio (ATR). The study concluded that the influence of tangible non-current assets on the financial performance of the manufacturing companies is more than that of the intangible non- current assets in Nigeria and therefore recommended that attention should be paid to optimum asset utilization by the manufacturing firms in Nigeria. The study also recommended that manufacturing firms in Nigeria can leverage on debts to fund their assets.