The term "microbiota" refers to the complex, delicately balanced ecological community that makes up the human body and contains symbiotic and pathogenic bacteria. The gut-brain-immune axis, a bidirectional communication route, is a vital component of this system of living beings in integrating the nervous, endocrine, and immune systems. Despite the wealth of research in human medicine, the relationship between unusual behavioural phenotypes and changes in animals gut microbiota has received little attention in the literature in the past. The scientific word for conventional animal healthcare is ethno veterinary medicine, which offers affordable options to allopathic medications. In this research work we prepared a remedy (Digestovet bolus) effective for all gastrointestinal issues, including anorexia, indigestion, off-feed, a lack of ruminating, impaction, etc. Digestovet Bolus is traditionally given orally to working bullocks to treat anorexia and off-feed. It works well for digestive disorders, diarrhoea, and impaction, lack of rumination, repeated breeding/reproductive issues, and internal parasites. It is additionally utilized as an immunity booster to stave off illnesses including blue tongue, fever, mastitis, and hemorrhagic sepsis.