About Us   |   Contact Us   |  
Submission  

Assessment of OqxA and OqxB Resistance Genes in Escherichia coli and Klebsiella pnuemoniae Clinical Isolates Based on Polymerase Chain Reaction from Niger Delta University Teaching Hospital, Yenagoa, Bayelsa State

DOI : https://doi.org/10.36349/easms.2023.v06i04.005
PDF
HTML
XML

Antimicrobial resistance continues to pose serious public health challenges. OqxA and OqxB are multidrug resistance genes that confer Escherichia coli and Klebsiella pneumoniae resistance to more than one antibiotics. The purpose of this study was to detect OqxA in Escherichia coli and OqxB in Klebsiella pneumoniae from clinical samples isolated from Niger Delta University, Yenagoa. A total of 50 samples were collected. The bacterial isolates were identified using a standard bacteriological technique, the genes were detected using Polymerase Chain Reaction while the antibiotic susceptibility testing was done by disc diffusion. Of the 50 clinical isolates, 9(18%) were positive for E. coli while 15(30%) were positive for Klebsiella pnuemoniae. The total number of isolates were 18(36%) from male and 32(64%) from females. The susceptibility pattern of the isolates revealed that Escherichia coli exhibited the highest resistance of 100% to Cefuroxime and Augmentin, followed by Gentamycin and Ofloxacin (.55%) and Ciprofloxacin (11.11%) while Klebsiella pneumoniae Nalidixic Acid, Augmentin Cephalexin and Sulfamethoxazole shows 100% resistance respectively. Of the 9 that were E.coli isolates, 8(88.9%) harboured OqX A while of the 15 that were Klebsiella pneumoniae isolates, 13 (86.7%) harboured the OqxB genes. There was 100% resistance to Nalidixic acid, cefuroxime and sulfamethoxazole, with lowest resitance to meropenem (46.7%) and ciprofloxacin (60%). The report clearly demonstrates an urgent need for surveillance against these bacteria especially as they are pathogens of public health concerns to minimize the increasing pace of multidrug resistance conferred on these bacteria by OxAB genes.

TOP EDITORS

OPEN ACCESS JOURNALS

Dr. Afroza Begum

Lecturer, Dept. of Pharmacology and Therapeutics, Shaheed Monsur Ali Medical College & Hospital, Uttara, Dhaka-1230, Bangladesh

BEST AUTHOR

Of The Month

TRACK YOUR ARTICLE

Enter the Manuscript Reference Number (MRN)
Get Details

Contact us


EAS Publisher (East African Scholars Publisher)
Nairobi, Kenya


Phone : +91-9365665504
Whatsapp : +91-8724002629
Email : easpublisher@gmail.com

About Us


EAS Publisher (East African Scholars Publisher) is an international scholar’s publisher for open access scientific journals in both print and online publishing from Kenya. Its aim is to provide scholars ... Read More Here

*This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2020, All Rights Reserved | SASPR Edu International Pvt. Ltd.

Developed by JM