Background: Global e-commerce exists. E-commerce is essential to the economy of emerging nations. Studies on e-commerce innovation abound, but there are less on government electronic payments as part of new or inventive payment agendas. Objective: To analyze the contribution of E-commerce in Business sector in Bangladesh. Materials and Methods: Both qualitative and quantitative research methodologies were used. In this study, total 45 for e-commerce users and 45 for e-commerce producers were chosen for surveys with structured questions and 45 for interviews with unstructured questions and observations. A simple descriptive analysis was conducted for this study. Using Microsoft Excel, the required findings were shown as tables, charts, and graphs. Results: Most of the service provider (93.7%) and service receiver (96.5%) said that the contribution of e-commerce business is positive and (43.2%) service providers said that current position of e-commerce business in Bangladesh is very good on the other hand (26.6%) service receiver said very good. Conclusion: E-commerce has significantly improved commercial operations in Bangladesh and modernized conventional commerce with a contemporary e-commerce approach.