Background: Cardiovascular diseases (CVD) are the commonest cause of mortality and morbidity worldwide. Despite the rising proportions of CAD, only limited data are available on the relationship between Vitamin D. This study was aimed to assess the serum levels of 25(OH) Vitamin D and its causal association of Vitamin D deficiency as an independent risk factor in patients with Acute ST-Elevation Myocardial Infarction in National Institute of Medical Sciences and Research, Jaipur, Rajasthan. Material and Methods: Patients admitted with acute ST- Elevation and NON-ST- Elevation Myocardial Infarction in the CCU unit of the National Institute of Medical Science & Research, Jaipur were chosen as cases. Rest healthy same age and sex-match ambulatory subjects were kept as controls. The data entered in the MS EXCEL spreadsheet and analysis will be done using Statistical Package for Social Sciences (SPSS) version 23.0. Continuous variables were presented as mean ± SD. Categorical variables were expressed as frequencies and percentages. The Chi-square test of association or Fischer exact test has been used to determine if there is an association between two categorical variables. Results: Among 50 study cases, the prevalent age group where 50-60 years was 16(32%) followed by 40-50 years (28%) while in control the majority of the participants were in between 40-50 years, 16(32%) followed by 15 cases (30%) in 30-40 years. It was observed that the mean BMI of the study cases was 29.11 ± 3.1 and for controls was 24.41 ± 1.92. Fifty-four percent of cases were found overweight with a BMI range between 25-29.9 and 21(42%) were found obese. In considering the control group, 21(42%) subjects were found overweight with a BMI range between 25-29.9 while no subject was obese with above 30. Most of the cases with vitamin deficiency and vitamin insufficiency were either overweight or obese and had BMI above 25 and a statistically significant difference with p=0.02828. ......