Introduction: Anemia is a common disorder in India, although there are plenty of reasons attributable to Anemia. Studies are reporting the beneficial effect of Anemia on lipid profile. Aim: This research was conducted to study the pattern of lipid profiles in anemic patients. Material and Method: Present hospital-based cross-sectional study was conducted among patients more than 18yrs of age attending the OPD or admitted to the medicine department National Institute of Medical Sciences and Research, Jaipur, with diagnosed Anemia. The study was done over a period of two years on patients with proven cases of Anemia. After informed & written consent from the patients, detailed history, clinical assessment & laboratory investigation was done. Results: In the present study a total of 280 patients fulfilling inclusion criteria are included in the study. The mean age of the patients was found to be 39.44±14.92yrs; among them, 60% were female, and 40% were male patients with female preponderance. There was significant positive strength of association of hemoglobin with the lipid profile, which included the total cholesterol, LDL, HDL, triglycerides, and VLDL cholesterol l(p<0.05). Conclusion: The present study showed a significant strength of association between the hemoglobin level and the lipid profile parameters. Also, the lower mean level of lipid parameters with the severity of grade of Anemia.