Background: The orbital aperture morphometry can be considered as a valuable tool in gender determination since orbit possesses resistance to damage and disintegration processes. Aim and Objective: The aim and objective of this study was to evaluate the orbital aperture dimensions in nalgonda population and verify their relationship with gender. Materials and Methods: The present retrospective study was conducted to evaluate the morphometric dimensions of orbital aperture seen on digital orthopantomogram taken using PLANMECA digital machine and ROMEXIS software. The height and width of the orbits were measured using measuring tools in the accompanying software. Data were analyzed using IBM SPSS Statistics for Windows, Version 23.0. Armonk, NY: IBM Corp. Results: Linear measurements such as orbital height and orbital width, were significantly greater in males than females in the Nalgonda population with P < 0.001. The present study found 85.6% accuracy after subjecting the obtained value to discriminant function analysis. Conclusion: The discriminant scores greater than 1.5 value indicate the sample as male and scores lesser than 1.5 value indicate the sample as female. Therefore, orbital aperture measurements can be used for gender determination in human identification.