Post-menopause is caused by the decreasing estrogen resulting in menopausal symptoms. Menopause Rating Scale (MRS) is a standardized questionnaire used to evaluate the menopausal symptoms. Vitamin D is believed relieving the menopausal symptoms. The purpose of this research is to figure out the relationship between 25(OH)D and menopausal symptoms based on MRS.This research employed an observational research method with a cross sectional study. The research population was all post-menopausal patients at Dr. Saiful Anwar Hospital, Malang in March 2020. The inclusion criteria are post-menopausal patients aged >45 years old. The inclusion criteria were the patients having the steroid medication, experiencing autoimmune and thyroid abnormality. The total research subjects were 39 respondents. The 25(OH)D level was examined using ELISA.The results showed that 8(20.5%) deficiency, 22(56,4%) insufficiency, and 9(23,1%) sufficiency. Based on MRS, 8 (20.5%) mild, 12(30.8%) moderate, 17(43.6%) severe, 2(5,1%) very severe menopausal symptoms. Those symptoms were divided into 3 scales: somatic scale moderately experienced by 16 (41%), psychological scale moderately experienced by 21(53.8%), and urogenital scale severely experienced by 17(43.6%) respondents. The results obtained from the spearman correlational analysis showed that there are significant and non-linear relationship of 25(OH)D with somatic symptoms (r -0.652; p 0.000), with psychological symptoms (r -0.452; p 0.004), urogenital symptoms (r -0.368; p 0.021), and with the total MRS scale (r -0.712; p 0.000). The concludes, there is a significant and negative relationship between 25(OH)D and menopausal symptoms based on MRS and close relationship respectively from the strongest to the weakest symptoms (somatic-psychological-urogenital)