Background: Students as social control means that in any situation they must be able to control the movement of people to be wiser in receiving various information, one of which is regarding the COVID-19 vaccine so that they are expected to have good knowledge and attitudes towards COVID-19 vaccination to be able to educate the public to increase awareness and vigilance against COVID-19 through vaccination. Research Objective: To find out whether there are differences in knowledge and attitudes towards COVID-19 vaccination among Health and Non-Health Students at the Universitas Nusa Cendana Settings and Design: This is a cross-sectional approach with a comparative analysis design. Method and Material: Respondents involved were students of the Faculty of Medicine, Public Health, Law, and Economics & Business. There are 136 respondents who met the inclusion criteria that were chosen through the simple random sampling method using validated and reliable questionnaires. Statistical Analysis Used: The research was analyzed univariately using a frequency distribution table and bivariate analysis using the Kolmogorov Smirnov test with the test results for the level of knowledge obtained p-value = 0.864 and attitude obtained p-value =1,000. The level of knowledge of most health students is good with a percentage of 100% while non-health students are categorized as good at 89.7%, 8.8% enough, and 1.5% less. The attitude of health students was in the good category of 95.6% and 4.4% enough, while non-health students were in the good category of 89.7%, 7.4% enough, and 2.9% less. Conclusion: There are no differences in knowledge and attitudes towards COVID-19 vaccination in health and non-health students at the Universitas Nusa Cendana.