The poor outcome of labour and delivery in sub-Saharan Africa could be related to inadequate knowledge of the partograph. The partograph is an afore-printed paper form on which the outcome of labour monitoring is recorded to assist in reducing maternal and fetal mortality. Hence, this study assessed the effect of nursing intervention on the knowledge of partograph among obstetric care providers in Primary Health Care Centers of Gwagwalada, Abuja. This study adopted a one group pretest-posttest quasi-experimental design also intended to train the trainers in the knowledge of partograph. Purposive sampling technique was used to select 64 participants from the 32 Primary Health care Centers. Socio-demographic questionnaire and test paper on knowledge of partograph (TP-KP) were used to collect data. A total of 59 and 58 obstetric care providers participated in the pre and post-test. A training module was developed for nursing intervention. Data obtained were analyzed using the Statistical Package for the Social Sciences (SPSS) version 21. Descriptive analysis provided answers to the research questions. Inferential statistics; independent t-test and ANOVA were used to provide answers to the hypotheses. Findings revealed that the pre-intervention mean score of knowledge of partograph was moderate (13.27 ± 4.31) and high (23.69 ± 3.19) post intervention. Results also showed significant difference in the pre and post intervention mean scores of all the obstetric care providers on knowledge of partograph (p=0.000) and between pre and post-intervention mean scores on knowledge of partograph among various cadres of obstetric care providers (p=0.000).