Background: Evidence based practice involves the utilization of proved findings from researches with clinical experience and patient preferences. Peripheral intravenous catheters are used in up to 60% of the pediatric patients. Research design and aim: this study was a descriptive study aimed to assess the knowledge and practices of pediatric nurses regarding evidence-based care of peripheral intravenous catheter in hospitalized children. Setting: the study was conducted at the pediatric medical inpatient department, Suez Canal University and Ismailia General Hospitals. The sample: included a convenient sample of all nurses (32) working in the above-mentioned settings. Data were gathered using an assessment sheet and observational checklists for the PIVC care. Results: revealed that (31.3%) of the studied nurses had satisfactory total score of knowledge, while (37.5%) of the studied nurses had satisfactory total score of practices regarding evidence-based care of peripheral intravenous catheter in hospitalized children. Conclusion: the study concluded that almost less than one third of the studied nurses had satisfactory total score of knowledge and more than one third of them had satisfactory total score of practices regarding evidence-based care of peripheral intravenous catheter in hospitalized children. Recommendation: raising the awareness of nurses regarding evidence-based care of peripheral intravenous catheter in hospitalized children.