Background: Infection control measures are essential for the containment of surgical infections. The current Operating Room (OR) should therefore, have well-developed policies for managing infections. The efficacy of those policies depends on how well they are adhered to by OR staff. Thus nurses ought to have great knowledge and strict adherence to the prevention of infections. Aim: The study aimed to study nurse's awareness of infection control measures in the operating room. Methods: A cross sectional study was carried out between June and august 2018 in port said general hospital. Data were collected for a purposive sample of all nurses (140), who work at the operating room. The instrument was used to collect study data include nurses’ socio-demographic and nurses knowledge as infection control principles and knowledge base related to the principles of asepsis .Results: Most of the studied nurses were female, more than one third of them in the age group 26-34 years, nearly half of them had bachelor’s degree and work as staff nurses for 1-3 years working experience in operating theatre. Shows that there are a highly Mean ± SD of nurse's overall knowledge scores regarding infection control measures were found in items related to infection control basis. While highly significant relation were found between nurse's sociodemographic characteristics and their knowledge regarding basis of sterilization and principles ,infection control basics mainly in items related to marital status , educational level. Conclusion: Despite the good knowledge and awareness of infection control measures that founded in operation room than knowledge in basis of sterilization and principles. We can concluded that there are a gab in infection control measures application regarding sterilization basis and principles. Recommendations: the study suggested that, continuous education training programs for nurses, and activating the role of infection control team. There are obvious needs to pos