Background: One of the most common complications of diabetes mellitus is diabetic retinopathy, which is preventable and treatable if it has early been detected. This study aimed to evaluate the effect of the educational program on diabetic patients’ knowledge and attitude regarding diabetes and diabetic retinopathy (DR).Methods: pre- test and post-test design was used at El rebat Center and Soba Diabetic Clinic. The participants were interviewed using structured questionnaire, and educational program. Forty seven patients completed the study. Data was collected and analyzed using SPSS version 16, (confidence level 0.95, and error margin 0.05). Results: Most of the respondents (63.8%) were females. The majority (53.19%) were in the age group 40-50 years. Housewives represented 63.8% and 39.13 of the participants had primary level of education. The duration of illness was 3-5 years in 44% of the participants and 90% of the participants have begun to develop diabetes complications. 66.6% of the participants had family history of diabetes mellitus.The test given two weeks after the educational program showed significant improvement in patients’ knowledge regarding diabetes (P_value 0.00) and diabetic retinopathy (P_value 0.00). Patients’ attitude towards eye examination significantly improved when evaluated two weeks after the educational program (P_value 0.00).during follow up a significant decrease in patients’ knowledge regarding diabetes and diabetic retinopathy was noted (P_value 0.00), and the attitude towards eye examination became significantly negative (P_valu 0.00).Conclusion: The instructional educational program brings positive change in diabetic patients’ knowledge and attitude regarding diabetes and diabetic retinopathy for short duration. For long term, adoption of worldwide educational programs and strategies will insure better out come.