The ability to utilize Typha grass as a potential substrate for bioethanol production was analyzed in this research, the sample of Typha grass collected from Kware Lake was pretreated by autoclaving after it was initially dried and grounded into powdered form. The pretreated samplewas enzymatically hydrolyzed using Aspergillus niger for 7 days. The hydrolyzate was used to produce bioethanol by fermentation using Saccharomyces cerevisiae which was determined by HPLC analysis.The results indicated that pretreated Typha grass sample that was having higher lignin and hemicellulose composition of 28.15 % and 30.0 % was reduced to 14.29 % and 7.8 % respectively. Also, the α-cellulose composition was increased to a highest composition of 23.2 % from a lowest composition of 10.0 %. The pretreated sample that was hydrolyzed produced a highest reducing sugar yield of 19.14 g/L and the fermented hydrolyzate produced a bioethanol concentration of 2.07 % at day 6 of the fermentation period. From the results of this research, it is concluded that bioethanol can be produced from Typha grass and pretreatment can lessen cristallinity and expose cellulose thereby escalating reducing sugar yield for maximum bioethanol production