The complexity associated with the patient's condition during the treatment process in the inpatient room makes them vulnerable to dangerous incidence. Therefore, productive safety culture is needed to reduce these complications with fewer side effects. This research therefore aims to determine the effect of the patient safety culture training program in Banda Aceh General Hospital (RSUD). This is a quasi-experimental research that uses the one-group pretest-posttest design. Data were obtained from a total of 55 nurses from the Neurology inpatient room using questionnaires on Survey Hospital On Patient Safety Culture (HSOPSC), before and after the training program. The obtained data were analyzed using descriptive statistics, Wilcoxon signed-rank test and paired t-test. The results showed an increase in nurses' perceptions of the 12 dimensions of patient safety culture before and after the training program (p-value = 0,000). In addition, for each dimension, there is an increase in teamwork with a p-value of 0,000 for manager's expectations, actions, support for patient safety, open communication, teamwork, staffing, handoffs/transitions, and non-punitive responses to errors. The feedback and communication errors had a p-value of 0.001, while the organizational learning and continuous improvement is 0.002), with a frequency of reported incidents at 0.003. In conclusion, the provision of training programs has the ability to improve the overall perception of patient safety culture. However, additional actions are needed, such as reporting incidents and non-punitive responses.