Covid-19 is an infectious disease caused by SARS-CoV-2. The behaviour of Grab Bike riders who do not follow health protocols can increase the spread of the Covid-19 virus. The initial survey of Covid-19 cases conducted by researchers with 10 riders Grab Bike Kupang found that 7 out of 10 Grab Bike riders had tested positive for Covid-19. Based on researchers' observations in the field, it is still possible to find Grab Bike riders who have not fully implemented every health protocol. Good knowledge and attitude can encourage Grab Bike riders to take action in implementing the Covid-19 health protocol. This study aims to describe the knowledge, attitudes, and actions of Grab Bike riders in implementing the Covid-19 health protocol in Kupang City to prevent the spread of Covid-19. This type of research is quantitative research with a descriptive survey method. This research was conducted in the Grab Bike riders work area in Kupang City with a total sample of 62 people and using an accidental sampling technique. Based on the results of the study, it is known that the knowledge of Grab Bike riders is in the good category, namely 55 people (88.7%), the enough categories are 7 people (11.3%), and there are no people in the fewer categories (0.0%). The attitude of Grab Bike riders is in the positive category, namely 62 people (100.0%), and there are no people in the negative category (0.0%). The actions of Grab Bike riders are in the good category, namely 54 people (87.1%) and the less category are 8 people (12, 9%). It is hoped that Grab Bike riders will always follow the Covid-19 health protocol that has been established and seek out the most up-to-date information on measures to prevent the Covid-19 virus from spreading.