Level of liver marker enzymes AST, ALT and ALP and level some biochemical parameters Albumin, Urea and Creatinine were assayed in rats fed with aqueous extract of Hyphaene thebaica. This study was done to determine the hepatoprotective activity of aqueous extract of Hyphaene thebaica in paracetamol-induced liver injury albino rats. Acute toxicity study (LD50) was first evaluated in rats before the commencement of the main study. Twenty five albino rats weighing between 90-120g were used. They were divided into five (5) groups of five (5) rats each. Rats in group one served as the normal control, group two as positive group they were treated with standard drug Silymarin at a dose of 50mg/kg, groups 3 and 4 were administered the aqueous extract daily by intubation for seven days at 250mg/kg for group and 500mg/kg for group four respectively. All treatments were carried out for 7 days and PCM intoxication was done 3 hours after the last dose. All animals were euthanized twenty four (24) hours after the last treatment; blood was collected, and used for estimation of biochemical parameters such as liver enzymes (alanine amino transferase (ALT), aspartate amino transferase (AST), and alkaline phosphatase (ALP), and level of serum albumin, Creatinine and urea. In the experiment carried out there was no significant difference (P>0.05) in terms of ALP of all the groups in comparison to the control group. Aqueous extract of H. thebaica however showed significant increase at P<0.05 in this protein, both urea and creatinine levels were increased significantly by PCM but were both decreased significantly by the administration of aqueous extract of H. thebaica.