EAS Publisher | Characterization of Rhodanese Extracted from Synodontis schall Liver

Characterization of Rhodanese Extracted from Synodontis schall Liver

DOI : https://doi.org/10.36349/easjnfs.2022.v04i02.004

1051 Downloads 18 Views

Rhodanese, a cyanide detoxifying enzyme was extracted from the liver of Synodontis schall and its characteristics investigated. Crude enzyme preparation was prepared and the enzyme was assayed by measuring the activity of rhodanese in RU min-1 mg-1. The results revealed that rhodanese extracted from Synodontis schall liver had km values for Na2S2O3 and KCN were 12.23mM±1.36 and 8.45mM±1.05 respectively. The enzyme had higher affinity for KCN than Na2S2O3. Dithio oxiamide, 2-mercaptoethanol and sodium metabisulfite were not capable of replacing Na2S2O3 as sulfur donors. Na2S2O3 had the highest relative activity followed by ammonium sulphate. Synodontis schall liver rhodanese had optimum activity at pH 8.0 and 45ºC. Relative activities of cations tested showed that none had any significant effect on Synodontis schall liver rhodanese. Rhodanese present in the liver of Synodontis schall had properties similar to those from other sources.

TOP EDITORS

Dr. T. Selvankumar
Chief Editor
EAS Journal of Biotechnology and Genetics

Dr. James Kay, PhD
Chief Editor
EAS Journal of Psychology and Behavioural Sciences

Dr. Rejeesh Menon
Chief Editor
EAS Journal of Medicine and Surgery

Dr. S. Jayachandran
Chief Editor
EAS Journal of Dentistry and Oral Medicine

Dr. Md. Habibur Rahman
Chief Editor
EAS Journal of Pharmacy and Pharmacology

Dr. Benard Chemwei, PhD
Chief Editor
East African Scholars Multidisciplinary Bulletin

NFI Joseph Lon
Chief Editor
EAS Journal of Humanities and Cultural Studies

Prof. Dr. Nazir Ahmad Suhail
Chief Editor
East African Scholar Journal of Engineering and Computer Sciences

Dr. Hamid Osman Hamid
Chief Editor
EAS Journals of Radiology and Imaging Technology

Dr. BOUCENNA Mounir
Chief Editor
EAS Journal of Veterinary Medical Science

OPEN ACCESS JOURNALS

Dr. Afroza Begum

Lecturer, Dept. of Pharmacology and Therapeutics, Shaheed Monsur Ali Medical College & Hospital, Uttara, Dhaka-1230, Bangladesh

BEST AUTHOR

Of The Month

TRACK YOUR ARTICLE

Enter the Manuscript Reference Number (MRN)

Get Details