Anxiety in pregnant women is an emotional response to the intellectual assessment of the danger ahead of the delivery process. This study aims to determine the factors associated with anxiety third trimester pregnant women in the face of deliveries at Health Center of Kassi-Kassi Rappocini District, Makassar City. This study design was cross-sectional study. Thetotal sample of 230 pregnant women in Health Center (Puskesmas)Kassi-Kassi RappociniDistrict of Makassar. Sampling was done by simple random sampling method. Data were analyzed using Chi Square test and logistic regression. Results of univariate analysis showedmost respondents aged 26-35 years (48.7%), while the least respondents aged over 36 years (126%). The results of the bivariate analysis also shows that there is arelationship between family support (p = 0049) and knowledge (p = 0.046) with anxiety third trimester pregnant women in the face of labor at health center of Kassi-Kassi Rappocini District of Makassar.The results of the bivariate showed no relationship between age (p = 0705), parity (p = 0.775) with anxiety third trimester pregnant women in the face of labor. The results of the bivariate analysis also shows that there is a relationship between family support (p = 0049) and knowledge (p = 0.046) with anxiety third trimester pregnant women in the face oflabor at health center of Kassi-Kassi Rappocini District of Makassar. Multivariate analysis showed knowledge variable (p <0.05) the most influence on anxiety third trimester pregnantwomen in the face of labor at health center of Kassi-Kassi Rappocini District ofMakassar. It was concluded that the factors associated with anxiety third trimester pregnantwomen in the face of labor in sub-district Puskesmas Kassi-Kassi Rappocini Makassar is family support and knowledge.