Introduction: MRSA is one the most common cause of nosocomial infections. Decolonization of MRSA from the anterior nares by mupirocin ointment is most commonly performed modality to prevent the transmission of infection among patients and health care personnel. Unprecedented use has led to development of resistance to mupirocin. The present study is conducted to determine the prevalence of mupirocin resistant S. aureus to understand the risk of transmission of nosocomial infections due to S. aureus in our health care setting Materials and methods: All clinical isolates of S. aureus obtained from various specimens sent for routine culture and sensitivity in the laboratory was included in the study. Methicillin resistance was determined by using cefoxitin disc 30 μg as per Clinical and Laboratory Standards Institute (CLSI) guidelines. Mupirocin resistance among S. aureus was determined by disk diffusion method using mupirocin discs of 5 μg and 200 μg. Results: Out of 310 S. aureus isolates, 141 isolates were MRSA. Mupirocin resistance among the MRSA isolates was found in 9 (6.4%) isolates. Among the mupirocin resistant strains, none of the isolates showed low-level of mupirocin resistance. Conclusion: Routine nasal decolonisation of health care personnel and surveillance of mupirocin resistance among the isolates obtained from the patients and the health care personnel should be done to prevent the transmission of infections.