Every health service must have a goal so that the highest level of health can be achieved. One of the health service facilities that have a goal is the puskesmas. Puskesmas is a health service facility that organizes primary health efforts, namely health efforts that become the community's first contact with health services. This study was conducted to determine the relationship between education level, occupation, accessibility, facilities and facilities, staff attitudes, and family support with the utilization of health services at the Naioni Community Health Center, Alak District. The type of research used is descriptive research with a quantitative approach. The method used is a cross sectional study. To obtain data in the field is done by interviewing using a questionnaire to 99 respondents and taken by cluster sampling. Respondents in this study were residents who were in the working area of the Naioni Community Health Center which consisted of 3 villages, namely Naioni Village (18 respondents), Manulai II Village (46 respondents), and Batuplat Village (35 respondents). The results of the study were analyzed using thetest chi-square showing that there is a relationship between accessibility (p = 0.000), facilities and facilities (p = 0.007), attitude of officers (p = 0.000), and family support (p = 0.000) with the utilization of health services in Naioni Community Health Center. While the variables of occupation (p = 0.325) and education (p = 0.258) did not have a relationship with the utilization of health services at the Naioni Health Center, Alak District, Kupang City.