Background: Pulmonary tuberculosis (TB) is an infectious disease that is still a major health problem in Indonesia. The duration of TB treatment is 6-8 months, the long treatment period for TB disease causes many TB sufferers to be lazy to do routine treatment because they feel bored, tired, saturated, and depressed, causing stress and ultimately treatment is not optimal. Stress that is not handled properly can have a negative impact. There are several things that affect stress in individuals. One that affects stress is gender. Objective: To analyze the relationship between sex and length of treatment on stress in pulmonary tuberculosis patients at Oesapa Health Center, Kupang City. Methods: This study was an observational analytic study with a cross sectional study design conducted on pulmonary tuberculosis patients at Oesapa Health Center, Kupang City by filling in the Kessler Psychological Distress Scale (K-10) questionnaire. The sample in this study was 30 people. This research was analyzed by univariate, bivariate using the Chi-Square test. Results: The results of the Chi-Square Tests showed a significant value between sex and stress of pulmonary TB patients at Oesapa Health Center with a value of p = 0.548 or p> 0.05, a significant level between length of treatment and stress of pulmonary TB patients at Oesapa puskesmas = 0.681 or p> 0.05, which means that there is no relationship between length of treatment and stress in pulmonary TB patients at Oesapa puskesmas. Conclusion: There is no relationship between gender and length of treatment with stress in pulmonary tuberculosis patients at Oesapa Health Center.