With recent research in novel drug delivery formulations to provide stable and economical drug delivery systems, the pivot is on hydrogel which are known to minimize the problems of not only conventional dosage forms but also of novel drug delivery systems. Hydrogel is a biocompatible, convenient and stable drug delivery system for molecules as small as NSAIDs (Non-steroidal anti-inflammatory drugs) or as large as proteins and peptides. Healing of wounds begins from the moment of injury and can be extended for varying periods of time depending on the degree of wounding and the process of wound healing can be broadly classified into three stages; inflammatory phase, proliferate phase, and finally the remodeling phase which finally governed the strength and appearance of the healed tissues. Research had been recognized flavonoids to be an exceptional class of therapeutic molecules due to their versatile therapeutic potential. Rutin, class of flavonoid (vitamin P) has been explored for a numeral of pharmacological effects. In the present work attempted was made to determine the efficacy of rutin in hydrogel drug delivery system for the evaluation of wound healing activity. The drug content of the formulation H2 (0.025%w/w) was less than H3 (0.030%w/w), therefore H2 formulation was considered as best formulation.