Background: The radiation risks to workers, public and to the environment that may arise from unsafe practices have to be assessed and, if necessary, controlled. Objective: The study is aimed to assess procedural radiation protection measures and to explore challenges faced by radio-diagnostic centres on safety operations of radiation protection practices. Method: A prospective cross-sectional survey was conducted among 26 radio-diagnostic workers in some centres in Gombe State, Nigeria. The questionnaire consists of three sections which include socio-demographic and work related information, procedural safety and safety operations. The data were analyzed using SPSS version 20.0 and descriptive statistics such as frequency and percentage were obtained. Results: More than two-third of the respondents, 61.5% (n=16) closed their x-ray room door during exposure and majority of the respondents, 92.3% (n=24) always move the patient’s feet away from the couch or table during positioning of the ambulant patient for upper extremity. Those respondents that use gonadal shield on patient’s were 26.9% (n=7). Majority of the respondents 76.9% (n=20) don’t have a radiation safety officer/radiation safety adviser (RSO/RSA) at their facility. More than two-third of the respondents, 80.8% (n=21) have never attended any training. All the participants 100% (n=26) have no radiation exposure monitoring device at their facility. Conclusion: Procedural safety was adequate in all the facilities, except for the non-usage of the gonad shied by almost all of the respondents, while safety operations are poor in most of the radio-diagnostic centres.