Physiologically the female bovine is considered non-seasonal polyestric. The beginning of the estrous cycle occurs when the female enters puberty, however, the age at which estrus begins is variable. There are specific protocols for inducing puberty in super precocious heifers that favor reproductive efficiency in the herd, increasing estrus and conception rates. Therefore, the aim of this study was to evaluate puberty induction and fixed-time artificial insemination in super precocious heifers from a farm in southern Bahia. For this, 334 F1 heifers, crossing Aberdeen Angus with Nellore had puberty induced by the administration of injectable progesterone, reproductive vaccine CattleMaster Gold® and Fosfosal® and after twelve days, estradiol cypionate was administered. Finally, artificial insemination for a fixed time is performed. It was observed that the conception rate of the animals that participated in the experiment carried out was 69,76% of initial pregnancy in their first pregnancy diagnosis, while in the second evaluation the result was 67,06% of pregnant animals, consisting of a loss embryonic 2,7%. Thus, a satisfactory result of the association between puberty induction and fixed-time artificial insemination was observed. Further studies comparing this protocol with the conventional technique are needed to verify the best approach.