Background: Kupang Regency and Kupang City are two areas with a high number of infant mortality cases (including AKN) and contribute quite significantly to the number of infant and neonatal deaths at provincial and national levels and make both regions one of the regions with the number of infant and neonatal deaths is higher than other regions in Indonesia. Method: This research is a non-experimental study with a cross sectional approach. The research sample was 73 midwives in all puskesmas in Kupa City and Kupang Regency which were determined by total sampling. Result: The results showed that (a) the knowledge factor did not have a relationship with the skills of the midwife in carrying out resuscitation and stabilization in neonates who experienced respiratory distress in the delivery room of the Kupang area health center. (b) The training factor has a relationship with the skills of the midwife in carrying out resuscitation and stabilization in neonates who experience respiratory distress in the delivery room of the Kupang area health center. (c) The length of service factor has a relationship with the skills of the midwife in carrying out resuscitation and stabilization in neonates who experience respiratory distress in the delivery room of the Kupang area health center. (d) The work status factor does not have a relationship with the skills of the midwife in carrying out resuscitation and stabilization in neonates who experience respiratory distress in the delivery room of the Kupang area health center. (e) The factor of tenure is the factor that has the most dominant relationship with the skills of the midwife.