Myocardial infarction and related cardiovascular complications are the main causes of deaths throughout the world. The use of herbal antioxidants is increasing as defensive agents against number of cardiovascular abnormalities. The bioactive agents from natural sources have gained fundamental importance in modern system of medicines, reducing the risks of cardiac ailments by scavenging the free radicals formation. Phoenix dactylifera L. is a fruit bearing tree with a lot of prospects. Its fruits, and seeds otherwise known as pit and by products are made up of nutritional and medicinal potentials. The ethanolic extract of Phoenix dactylifera fruit was studied for the cardio protective and antioxidant activity against Isoproterenol induced myocardial infarction in wistar albino rats. Phoenix dactylifera showed significant cardio protective effect by reversing the cardio biomarkers (CK-MB, LDH) and oxidative stress markers (SOD, GSH, GPX, CAT, LPO) induced by Isoproterenol. The findings of cardio biomarkers from serum and oxidative stress markers from myocardial tissue in the present work are comparable with the reference control Vitamin E. The results conclude that ethanolic extract of Phoenix dactylifera showed cardio protective activity and the effect may be mediated by its antioxidant potential.