Introduction: Burnout syndrome is a condition in which a person suffers emotional exhaustion, a loss of respect or a positive perspective of others, and a reduction in self-achievement as a result of prolonged stress. The aim of this study was to analyze the causes of burnout syndrome among nurses at the Mgr. Gabriel Manek, SVD Regional Public Hospital Atambua. Methods: A quantitative cross-sectional method was employed for the research. Based on the specified exclusion criteria, the sample size for this study was 104 individuals. After receiving an ethical permit, primary and secondary data were collected from September to October 2020. Multiple logistic regression was utilized in the data analysis technique. Result: Based on logistic regression analysis, it was found that individual factors had an impact on the incidence of burnout, with a p value of 0.0320.05. The incidence of burnout is influenced by organizational factors, with a p value of 0.0480.05. The findings of logistic regression analysis showed the effect of organizational factors on the incidence of burnout, with a p value of 0.018 0.05. The calculation findings reveal that the magnitude of the three variables' effect on burnout is 0.0122 or 1.22 percent. This indicates that individual factors, organizational factors, and the respondent's work environment will influence the incidence of burnout by 1.22 percent. Conclusion: The incidence of burnout syndrome is simultaneously affected by individual, organizational, and work environments.