This research aimed to analyze the effect of transport media and post-mortem storage time on the quality of Bali mongrel dogs spermatozoa. Coconut water and saline solution were used as the transport medium for the epididymal samples, and sperm quality parameter analysis was carried out as well as after one hour and two hours. Testicles from 18 (2-4 years old) mongrel dogs were collected via a castration program were used in this study. From each dog, the testicles were transported in different transported media to the laboratory. One testicle was maintained in sterile saline solution, where the other testicle was in coconut water, and sperm quality parameter analysis was carried out as well as after one hour and two hours. Epididymal sperm was harvested through flushing using an egg yolk tris extender. The motility of sperm was evaluated by adding a drop of semen on a warm glass slide covered with a glass slip and viewed at a magnification of x40. Liveability and abnormality of sperm were assessed by the eosin–nigrosin stained technique. Motility and viable spermatozoa could be recovered after two hours in coconut water and saline solution, although motility decreases significantly after two-hour storage time. It is concluded that coconut water and saline solution have a protective effect on epididymal sperm. Motile and viable spermatozoa can be recovered from the epididymides after storage at transport media for two hours.