Licorice is an anti-spasmodic drug for treating gastrocnemius spasm, and it is well-known that licorice targets voltage-gated sodium channels (VGSCs). Herein, researchers studied the effect of G. inflata on Nav1.4 Na+ currents using CHO cells expressing human Nav1.4 VGSCs. On treating G. inflata, it is showed that 4 mg/ml hydroalcoholic extracts of G. inflata at 30%, 50%, 70% and 90% (v/v) acquired 100.00%, 96.70%, 32.14% and 48.18% inhibition on INav1.4. By contrast, at 8 mg/ml we found that at 30%, 50%, 70% and 90% (v/v), inhibition rates of 100.00%, 10.0%, 100.00% and 100.00% on INav1.4 were respectively seen. Treatment with echinatin, isoliquiritigenin, glycyrrhizic acid and liquiritin exhibited higher inhibition rates of 33.20%, 39.98%, 20.54% and 22.62% respectively. And the concentrations ranged from 0.30μg/ml, 0.17μg/ml, 16.74μg/ml and 43.70μg/ml to 1.12μg/ml, 0.32μg/ml, 39.47μg/ml, 122.58μg/ml. Nevertheless, neoisoliquiritin, glycyrrhetinic acid, formononetin, and liquiritigenin exhibited lower inhibition rates about 20%. Furthermore, treatment with isoliquiritin, liquiritin apioside and neoliquiritin had negligible effects on INav1.4. It is found that glycyrrhizic acid attained a maximum concentration of 122.58μg/ml, but echinatin achieved the lowest concentration. The hydroalcoholic extracts of G. inflata had obvious inhibitory effects on Nav1.4 VGSCs which may represent a very important mechanism in treating gastrocnemius. Meanwhile, it can also guide further study regarding the material basis and therapeutic mechanism for gastrocnemius spasm with a peony and licorice decoction and other Traditional Chinese Medicines containing glycyrrhiza.