Background: Gallstones, also widely recognized as cholelithiasis, cause histological changes including chronic inflammation, glandular hyperplasia, granulomatous inflammation, metaplasia dysplasia and anaplasia (Carcinoma). As more than just a result, the goal of this system was also to evaluate how gallstones mucosal changes limit the amount and number of stones in cholecystectomy patients. Methods: At Rajshahi Medical College Hospital and Private Hospital in, a multicenter non-randomized experimental prospective study comprising n=264 patients with symptomatic cholelithiasis who were scheduled to have laparoscopic cholecystectomy with written and informed consent between 2014 and 2020 was conducted. All of the patients underwent a preoperative biochemical profile, as well as a complete abdominal ultrasound, was performed by surgeons who were very trained and experienced. The morphologic profiles of gallstones were documented and examined. The mucosal tissues from the gallbladder were transported to a general pathology lab for analysis. All gallbladder mucosal specimens were histopathological typed, and the number and type of gallstones were correlated. Results: A total of n=264 gallbladder specimens were collected, with 192 (73%) from female patients and 72 (27%) from male patients (M: F ratio 2:6.4). Patients were on average 42.5 years old (range 22-70). In 57 patients (21.52%), the outer gallbladder surface was clogged, the wall thickness was raised in 86 (32.57%), and mucosal abnormalities were discovered in 121 individuals (45.91%). On microscopy, 155 specimens (58.36%) had epithelial hyperplasia, 44 (16.85%) had antral metaplasia, 41 (15.57%) had intestinal metaplasia, 22 (8.52%) had dysplasia, and one had cancer in situ 2 (0.7%). We have found the satisfactory (Excellent and good) result 96.24% and unsatisfactory (Post Cholecystectomy Syndrome) was 3.76%. When the amount and kind of stones were linked with the gallbladder mucosal response, .................